Evaluation of antibody responses to the early transcribed membrane protein family in Plasmodium vivax

Parasites & Vectors

Table 3 Prevalence, 95% confidence intervals, and mean fluorescence intensities of IgG responses to PvETRAMPs in P. vivax malaria patients and healthy individuals

Antigen (P. vivax)	Sample (n)	No. of samples		Sensitivity (%)/Specificity (%)	95% CI (%)	MFI	Cut-off value^a	t-value	P-value^b
Antigen (P. vivax)	Sample (n)	Positive	Negative	Sensitivity (%)/Specificity (%)	95% CI (%)	MFI	Cut-off value^a	t-value	P-value^b
ETRAMP4	Patients (50)	31	19	62.0/97.5	48.2–74.1	10,713 ± 10,787	52,033	4.341	< 0.0001
ETRAMP4	Healthy (40)	1	39		87.1–99.6	3276 ± 963.4
ETRAMP5	Patients (50)	24	26	48.0/95.0	34.8–61.5	6115 ± 5629	4579	3.877	0.0002
ETRAMP5	Healthy (40)	2	38		83.5–98.6	2620 ± 979.4
ETRAMP8	Patients (50)	23	27	46.0/95.0	32.8–59.6	6678 ± 6376	4589	3.600	0.0005
ETRAMP8	Healthy (40)	2	38		83.5–98.6	3023 ± 783.1
ETRAMP9	Patients (32)	14	18	43.8/100	28.2–60.7	4428 ± 2008	3968	2.015	0.05
ETRAMP9	Healthy (8)	0	8		67.6–100	2974 ± 497.2
ETRAMP10.3	Patients (50)	20	30	40.0/97.5	27.6–53.8	5880 ± 5540	4259	3.643	0.0005
ETRAMP10.3	Healthy (40)	1	39		87.1–99.6	2659 ± 799.8
ETRAMP11.2	Patients (56)	43	13	76.8/97.5	64.2–85.9	12,647 ± 3326	9733	10.39	< 0.0001
ETRAMP11.2	Healthy (40)	1	39		87.1–99.5	6811 ± 1461

^aCut-off, the mean fluorescence intensity of the malaria-naïve samples plus 2 standard deviations
^bP-value, the difference in the total IgG level for each antigen between P. vivax malaria patients and healthy individuals was calculated with the unpaired t-test. A P-value of < 0.05 was considered statistically significant
Abbreviations: CI, confidence interval; MFI, mean fluorescence intensity

ISSN: 1756-3305