Fig. 2From: Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central AfricaMultiple amino acid sequence alignment of six Tp1 antigen variants in 116 T. parva samples obtained from DRC and Burundi. Antigen variants are named var-1 to var-34. The single letter amino acid code is used. The antigen variants nomenclature used in this study was first proposed by Pelle et al. [36]. Variants var-1 and var-3 were first described by Pelle et al. [36] and var-31 by Salih et al. [37]. The numbers in square brackets behind variants names indicate the number of T. parva isolates represented by each variant. The single previously identified T. parva CD8+ T cell target epitope is bolded and boxed. The polymorphic residues in the T cell epitope are coloured in red. Conserved amino acid residues are denoted by (*) below the alignment, and dashes (–) denote insertion region. Nested PCR primers used for sequencing are shaded and boxed in flanked regions. The Muguga sequence (GenBank: JF451936) was used as the reference sequence; it represents the other component of the Muguga cocktail vaccine (Serengeti-transformed and Kiambu 5) that are identical to Muguga strain sequence for the Tp1 locus. Tp1 antigen variant var-1 is found in the three Muguga vaccine strains. Corresponding gene alleles and sample characteristics are presented in Additional file 3: Table S3Back to article page