Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central Africa

Table 2 Tp1 and Tp2 sequence diversity in cattle-derived T. parva from RDC and Burundi

Gene locus	AEZ	Sample size	Nucleotide sequences			Amino acid sequences
Gene locus	AEZ	Sample size	Polymorphic sites^a	No. of alleles^b	Nucleotide diversity ± SD	Polymorphic sites^a	No. of antigen variants^b
Tp1	DRC AEZ1	31	12 + ind	7	0.008 ± 0.002	7 + ind	5
	DRC AEZ2	27	12 + ind	8	0.007 ± 0.002	7 + ind	6
	DRC AEZ3	25	0	1	0	0	1
	Burundi AEZ1	33	4	3	0.005 ± 0.0004	3	3
Overall Tp1		116	14 + ind	11	0.005 ± 0.0007	7 + ind	6
Tp2	DRC AEZ1	25	166	7	0.13 ± 0.025	82	7
	DRC AEZ2	20	165	5	0.17 ± 0.016	81	5
	DRC AEZ3	23	2	2	0.002 ± 0.0004	2	2
	Burundi AEZ1	28	175	6	0.16 ± 0.017	85	5
Overall Tp2		96	181	10	0.14 ± 0.01	88	9

^aThe insertion region (ind) was excluded for the determination of the number of polymorphic sites and the nucleotide diversity
^bAlleles represent distinct nucleotide sequences diverged at least by one substitution (Additional file 4: Figure S1, Additional file 8: Figure S2), while antigen variants represent predicted distinct protein sequences (Figs. 2, 4)
Abbreviations: AEZ, agro-ecological zone; ind, indels; SD, standard deviation

ISSN: 1756-3305