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Fig. 5 | Parasites & Vectors

Fig. 5

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Fig. 5

Average differences in Cq-values for quantitative PCR Plate (v) between Cryptosporidium parvum oocysts inoculated onto HCT-8 monolayer plate B, pure oocyst serial dilution B and equivalent template dilution B. Error bars represent the standard deviation of the mean (SD) from 3 biological repeats for cell culture data and SD of two technical repeats of the pure oocyst serial dilution and equivalent template dilution data. Asterisks indicate significantly increased Cq signal (P < 0.05) compared to cell culture-derived values

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