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Table 1 Cq values of quantitative PCR Plate (v)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Oocyst inoculate Well 1Well 2Well 3
 TR1TR2TR1TR2TR1TR2
100,000 31.7531.1130.7230.5530.4230
Mean ± SDTR31.43 ± 0.32 30.64 ± 0.09 30.21 ± 0.21 
BR30.76 ± 0.50     
10,000 31.4831.6631.1531.5431.8537.07
Mean ± SDTR31.57 ± 0.09 31.35 ± 0.19 32.46 ± 0.19 
BR31.79 ± 0.48     
1000 na37.5935.9635.6436.1435.57
Mean ± SDTRna 35.8 ± 0.16 35.86 ± 0.29 
BR36.18 ± 0.85     
100 39.24na38.34nana39.7
Mean ± SDTRna na na 
BRna     
Blank wells na43.42nana44.44na
Mean ± SDTRna     
BR43.92 ± 0.51     
Inactivated oocysts nana38.3336.32nana
Mean ± SDTRna 37.33 ± 1.00 nana
BR37.33 ± 1.00     
  1. Notes: Cryptosporidium parvum oocysts inoculated onto HCT-8 cell monolayer plate A. Two technical repeats for each well (biological repeat). Baseline threshold 11.89 RFU
  2. Abbreviations: TR, technical repeat; BR, biological repeat; SD, standard deviation; na, no DNA amplified