Table 1 Cq values of quantitative PCR Plate (v)
Oocyst inoculate | Well 1 | Well 2 | Well 3 | ||||
|---|---|---|---|---|---|---|---|
TR1 | TR2 | TR1 | TR2 | TR1 | TR2 | ||
100,000 | 31.75 | 31.11 | 30.72 | 30.55 | 30.42 | 30 | |
Mean ± SD | TR | 31.43 ± 0.32 | 30.64 ± 0.09 | 30.21 ± 0.21 | |||
BR | 30.76 ± 0.50 | ||||||
10,000 | 31.48 | 31.66 | 31.15 | 31.54 | 31.85 | 37.07 | |
Mean ± SD | TR | 31.57 ± 0.09 | 31.35 ± 0.19 | 32.46 ± 0.19 | |||
BR | 31.79 ± 0.48 | ||||||
1000 | na | 37.59 | 35.96 | 35.64 | 36.14 | 35.57 | |
Mean ± SD | TR | na | 35.8 ± 0.16 | 35.86 ± 0.29 | |||
BR | 36.18 ± 0.85 | ||||||
100 | 39.24 | na | 38.34 | na | na | 39.7 | |
Mean ± SD | TR | na | na | na | |||
BR | na | ||||||
Blank wells | na | 43.42 | na | na | 44.44 | na | |
Mean ± SD | TR | na | |||||
BR | 43.92 ± 0.51 | ||||||
Inactivated oocysts | na | na | 38.33 | 36.32 | na | na | |
Mean ± SD | TR | na | 37.33 ± 1.00 | na | na | ||
BR | 37.33 ± 1.00 | ||||||