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Table 2 Cq values of quantitative PCR Plate (v)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Oocyst quantity

TR1

TR2

1,000,000

29.29

28

Mean TR ± SD

28.65 ± 0.65

 

100,000

31.52

31.31

Mean TR ± SD

31.42 ± 0.11

 

10,000

34.16

34.53

Mean TR ± SD

34.35 ± 0.19

 

1000

37.11

37.49

Mean TR ± SD

37.3 ± 0.20

 

100

na

na

Mean TR ± SD

na

 

MQ

na

na

Mean TR ± SD

na

 
  1. Notes: Pure oocyst serial dilution A. Baseline threshold 11.89 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification
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Parasites & Vectors

ISSN: 1756-3305

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