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Table 2 Cq values of quantitative PCR Plate (v)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Oocyst quantityTR1TR2
1,000,00029.2928
Mean TR ± SD28.65 ± 0.65 
100,00031.5231.31
Mean TR ± SD31.42 ± 0.11 
10,00034.1634.53
Mean TR ± SD34.35 ± 0.19 
100037.1137.49
Mean TR ± SD37.3 ± 0.20 
100nana
Mean TR ± SDna 
MQnana
Mean TR ± SDna 
  1. Notes: Pure oocyst serial dilution A. Baseline threshold 11.89 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification