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Table 3 Cq values of quantitative PCR Plate (v)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Template dilutionTR1TR2
×1032.3631.64
Mean TR ± SD32 ± 0.36 
×10034.8434.95
Mean TR ± SD34.9 ± 0.06 
×1000nana
Mean TR ± SDna 
×10,000nana
Mean TR ± SDna 
  1. Notes: DNA template dilution of 106 oocyst suspension A. Baseline threshold 11.89 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification