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Table 3 Cq values of quantitative PCR Plate (v)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Template dilution

TR1

TR2

×10

32.36

31.64

Mean TR ± SD

32 ± 0.36

 

×100

34.84

34.95

Mean TR ± SD

34.9 ± 0.06

 

×1000

na

na

Mean TR ± SD

na

 

×10,000

na

na

Mean TR ± SD

na

 
  1. Notes: DNA template dilution of 106 oocyst suspension A. Baseline threshold 11.89 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification
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Parasites & Vectors

ISSN: 1756-3305

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