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Table 4 Cq values of Quantitative PCR Plate (vi)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Oocyst inoculate Well 1Well 2Well 3
 TR1TR2TR1TR2TR1TR2
100,000 30.2529.5228.2928.8328.428.98
Mean ± SDTR28.89 ± 0.37 29.06 ± 0.23 28.63 ± 0.29 
BR29.21 ± 0.50     
10,000 30.2430.9030.2930.3930.9531.19
Mean ± SDTR30.57 ± 0.33 30.34 ± 0.05 31.07 ± 0.12 
BR30.66 ± 0.30     
1000 36.6636.0934.7833.8234.5835.02
Mean ± SDTR36.38 ± 0.29 34.3 ± 0.48 34.8 ± 0.22 
BR35.16 ± 0.90     
100 35.8336.0637.637.7337.0438.64
Mean ± SDTR35.95 ± 0.12 37.67 ± 0.07 37.84 ± 0.80 
BR37.15 ± 0.86     
Blank wells na42.804441.2839.49na
Mean ± SDTRna 42.64 ± 1.36 na 
BR41.9 ± 1.69     
Inactivated oocysts 37.2337.4637.738.2336.6337.67
Mean ± SDTR37.35 ± 0.12 37.97 ± 0.27 37.15 ± 0.52 
BR37.49 ± 0.35     
  1. Notes: C. parvum oocysts inoculated onto HCT-8 cell monolayer plate B. Two technical repeats for each well (biological repeat). Baseline threshold 19.88 RFU
  2. Abbreviations: TR, technical repeat; BR, biological repeat; SD, standard deviation; na, no DNA amplification