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Table 6 Cq values of quantitative PCR Plate (vi)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Template dilutionTR1TR2
×1031.0730.96
Mean TR ± SD30.99 ± 0.03 
×10034.2834.58
Mean TR ± SD34.43 ± 0.20 
×100037.3737.4
Mean TR ± SD37.39 ± 0.02 
×10,000na41.49
Mean TR ± SDna 
  1. Notes: DNA template dilution of 106 oocyst suspension B. Baseline threshold 19.88 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification