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Table 6 Cq values of quantitative PCR Plate (vi)

From: Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

Template dilution

TR1

TR2

×10

31.07

30.96

Mean TR ± SD

30.99 ± 0.03

 

×100

34.28

34.58

Mean TR ± SD

34.43 ± 0.20

 

×1000

37.37

37.4

Mean TR ± SD

37.39 ± 0.02

 

×10,000

na

41.49

Mean TR ± SD

na

 
  1. Notes: DNA template dilution of 106 oocyst suspension B. Baseline threshold 19.88 RFU
  2. Abbreviations: TR, technical repeat; SD, standard deviation; na, no DNA amplification
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Parasites & Vectors

ISSN: 1756-3305

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