Fig. 4

Plasma membrane permeability assay results. RFU are the relative fluorescent units. A strong increase in the RFU will correspond with the internalization of the Sytox Green, this can occur only with the permeability is altered, enhancing > 500-fold the fluorescence of the dye upon nucleic acid binding. Error bars represent the standard deviations (SD). Each data point indicates the mean of the results of three measurements. Key: C-, negative control (parasites without treatment); C+, positive control (parasites permeabilized with Triton X-100)