Fig. 6

Serum cytokines levels after Galf treatment in L. donovani infected mice. Quantification of serum IL-1 β (a), IL-12p70 (b), IL-6 (c), IFN-γ (d), TNF-α (e), IL-17A (f), IL-27 (g) and MCP-1 (h) at the end of treatment (day 21). BALB/c mice were infected with L. donovani promastigotes, and treated from day 14 to day 21 with indicated treatments. Blood samples were collected at the end of treatment (day 21). Titrations were performed in duplicate using a bead-based immunoassay Legendplex® multi-analyte flow assay kit (BioLegend). Mean cytokine concentration ± SE (pg/ml) are represented by cross bars. Data are representative of one experiment. Each group contained 9 or 10 mice. Results from treated mice were compared to untreated mice using the non-parametric Kruskal–Wallis test (*P ≤ 0.05, **P ≤ 0.01)