Fig. 7
Myeloid cell attraction by Galf formulations and quantification of the granulomatous response in the liver. a Relative expression of MPO mRNA in liver according to treatment. b Immunohistochemical staining of MPO-positive cells in the liver. Representative microscopic fields according to treatment group. c Ratio of the granulomas surface by the total surface of liver section. d Number of small granulomas (< 50 cells) per mm2 of liver tissue. e Number of large granulomas (≥ 50 cells) per mm2 of liver tissue. Slide images were obtained using the NanoZoomer (Hamamatsu Photonics), and analyzed using the NDP.view2 viewing software (Hamamatsu Photonics) for granuloma measurement, quantification and size analysis. Each group contained 9 or 10 mice. Results from treated mice were compared to untreated mice using the non-parametric Kruskal–Wallis test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001)