Fig. 8

Organ weight and parasite burden according to treatment. Liver (a) and spleen (b) weight at sacrifice. CD8⁺ cell staining in the spleen from control mice (c), Lipo- (d), Galf- (e) and L-Galf- (f) treated mice. Parasite burdens in liver (g) and spleen (h). Leishmania donovani-infected mice were infected intraperitoneally with L. donovani promastigotes, treated from day 14 to day 21 with indicated treatments, and sacrificed 7 days after the end of treatment (day 28). After homogenization of standardized biopsies of liver and spleen, parasite loads were evaluated using a dilution assay. Results were expressed as the log of the titer, adjusted per milligram of organ. Means ± SE are represented by cross bars. Data are representative of one experiment. Each group contained 9 or 10 mice. Results from treated mice were compared to untreated mice using the non-parametric Kruskal–Wallis test (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001)