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Fig. 4 | Parasites & Vectors

Fig. 4

From: Molecular and physiological characterization of the chitin synthase B gene isolated from Culex pipiens pallens (Diptera: Culicidae)

Fig. 4

RNA interference (RNAi) of CpCHSB in third-instar larvae (n = 200). a Expression levels of CpCHSB at 72 h after injecting siCHSB assessed by RT-qPCR. b siCHSB injection into third-instar reduces body length in fourth-instar larvae, as well as midgut length (c). d Percentage of pupation (x-axis) after egg-hatching. e Comparison of wing length in wild type (WT), negative control (NC) and siCHSB adults. f Number of follicles per ovary and the number of eggs per female mosquito (g) after injecting (n = 50) siCHSB. All surviving individuals were used for measurements, and results are shown as the mean ± SE (Student’s t-tests: **P < 0.01, ***P < 0.001)

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