Fig. 5

CpCHSB gene suppression by RNAi at 1 day after injection (n = 200) in adult mosquitoes. a Expression levels of CpCHSB at 72 h after injecting siCHSB assessed by RT-qPCR. The group injected with siCHSB show a reduction in CpCHSB expression of 53% compared with the control group. b Relative chitin content at 72 h after siCHSB injection. c Midgut length at 72 h after siCHSB injection. d Number of follicles per ovary and number of eggs per female mosquito (e) after injecting siCHSB. Results are shown as the mean ± SE (Student’s t-tests; **P < 0.01, ***P < 0.001, ns, not significant)