Fig. 3

Simultaneous depletion of all three APL1 paralogs in Anopheles coluzzii does not cause mosquito morality. a Survival curves of An. coluzzii depleted for APL1 activity by dsAPL1 treatment (red lines) as compared to dsGFP controls (green lines), for sugar-fed mosquitoes. b Survival curves for mosquitoes fed a Plasmodium yoelii-infected blood meal. Survival curves from replicates are distinguished by line type (plain, dashed or dotted, respectively). X-axis indicates time after the start of recording, not mosquito age (see Methods). A Cox proportional hazards regression model was fitted to the data using treatment and replicate as predictor terms. The P-value associated with the dsRNA treatment term of the Cox model is shown on each panel. Panel a Wald statistic 0.95, df = 1, P = 0.34; Panel b Wald statistic = 1.589, df = 1, P = 0.112