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Fig. 2 | Parasites & Vectors

Fig. 2

From: Species-specific differences in Toxoplasma gondii, Neospora caninum and Besnoitia besnoiti seroprevalence in Namibian wildlife

Fig. 2

Immunoblots were used to confirm positive reactions in a Toxoplasma gondii TgSAG1 ELISA. The figure displays examples of T. gondii positive reactions in sera from Feliformia (African lion, brown hyena, caracal, cheetah, leopard and spotted hyena), Caniformia (wild dog, black-backed jackal and honey badger) and Ruminantia (blue wildebeest). Reactions to 5 antigen bands were recorded, based on Huskinson et al. [79]. A positive reaction with at least 2 of the 5 specific antigen bands (< 1–5) was regarded as a positive immunoblot reaction. Abbreviations: < 1, 55 kDa; < 2, 35 kDa; < 3, 30 kDa; < 4, 22 kDa; < 5, 6 kDa relative molecular weight (M (kDa)); Ink, India ink staining of antigen; Pf, Pc, Pr, positive controls for Feliformia, Caniformia and Ruminantia, respectively; Nf, Nc, Nr, negative controls for Feliformia, Caniformia and Ruminantia, respectively; Lion, African lion; Wild dog, African wild dog; Jackal, Black-backed jackal; H. badger, honey badger; Wildebeest, blue wildebeest

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