Fig. 3

Immunoblots were used to confirm positive reactions in a Neospora caninum NcSRS2 ELISA. The figure displays examples of N. caninum-positive reactions in sera from Caniformia (black-backed jackal) and Feliformia (brown hyena). Reactions to 5 antigen bands were recorded, based on Schares et al. [45]. A positive reaction with at least 2 of the 5 antigen bands (< 1–5) was regarded as a positive immunoblot reaction. Abbreviations: < 1, 37–38 kDa; < 2, 33 kDa; < 3, 30 kDa; < 4, 29 kDa; < 5, 17 kDa relative molecular weight (M (kDa)); Ink, India ink staining of antigen; Pc, Pf, positive controls for Caniformia and Feliformia, respectively; Nc, Nf, negative controls for Caniformia and Feliformia, respectively; Jackal, black-backed jackal; Br. hyena, brown hyena