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Fig. 4 | Parasites & Vectors

Fig. 4

From: Species-specific differences in Toxoplasma gondii, Neospora caninum and Besnoitia besnoiti seroprevalence in Namibian wildlife

Fig. 4

Immunoblots were used to confirm positive reactions in a Besnotia besnoiti ELISA (APure-BbELISA). The figure displays examples of B. besnoiti positive reactions in sera from Ruminantia (blue wildebeest) and Feliformia (African lion) against B. besnoiti tachyzoite (a) and bradyzoite (b) antigens. Reactions to 10 antigen bands were recorded, based on Schares et al. [47]. A positive reaction with at least 4 of the 10 antigen bands (< 1–10) was regarded as a positive immunoblot reaction. Abbreviations: M (kDa), reative molecular weight; Ink, India ink staining of antigen; Pr, Pf, positive controls for Ruminantia and Feliformia, respectively; Nr, Nf, negative controls for Ruminantia and Feliformia, respectively; Wildebeest, blue wildebeest; Lion, African lion

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