Fig. 2
Influence of PGRP-LA on gut microbiota in An. stephensi. a PGRP-LA silencing efficiency and specificity. Relative expression level of pgrp-la in dsLA mosquitoes was normalized to the gene’s expression in dsGFP controls. Error bars indicate standard error of the mean (n = 10). Results from one of three independent experiments are shown. Significance was determined by Student’s t-test (t(8) = 3.431, P = 0.0089). b Median culturable gut microbiota in dsRNA-treated mosquitoes. Each dot represents an individual mosquito and horizontal lines represent the medians. Significance was determined by Mann–Whitney test (U = 15.00, P = 0.0074). c Relative expression level of 16S in dsRNA-treated mosquitoes. Each dot represents an individual mosquito and horizontal lines represent the medians. Significance was determined by Mann–Whitney test (U = 7.00, P = 0.0070). d Relative expression levels of immune-related genes in dsRNA-treated, sugar-fed mosquitoes normalized to the gene’s expression in dsGFP controls. Error bars indicate standard error of the mean (n = 8). Results from one of three independent experiments are shown. Significance was determined by Student’s t-test (for details, see Additional file 3: Text S1). *P < 0.05, **P < 0.01