Highly specific PCR-RFLP assays for karyotyping the widespread 2Rb inversion in malaria vectors of the Anopheles gambiae complex

Table 1 PCR-RFLP genotyping assays for inversion 2Rb in An. gambiae and An. coluzzii

Tag position	Concord	Ref/Alt	Restriction enzyme	Chromosome cut	Primer pairs (5’-3’)	Amplicon size (bp)	Cleavage products (bp)
23170542	96.7%	C/T	DraIII	2R+^b	F: GCCGTTCTCCAGGCTCAG	481	348, 133
					R: AAACTCCATTGTACTGGCTGAA
26446866	96.7%	G/A	MspA1	2R+^b	F: TTCACAACGAAATGGCAAGA	463	264, 199
					R: TAGGGCAGTGTTGAGGGAAC
20247651	96.3%	G/A	TatI	2Rb	F: AATGGCCAGTCTCGAAAGAA	227	153, 74
					R: GAAGGGAACGTATGATAATGCAG

Abbreviations: Tag position, chromosome coordinate; Concord, minimum percent concordance with inversion genotype based on Love et al. [32]; Ref/Alt, reference and alternate allele at tag SNP

ISSN: 1756-3305