Multiplex PCRs for the specific identification of marsupial and deer species from faecal samples as a basis for non-invasive epidemiological studies of parasites

Table 1 Primers designed to target various portions of the mitochondrial cytochrome B gene (cytb) used to differentiate three marsupial and three deer species in two separate multiplex PCRs

Species	Primer	Sequence (5′-3′)	Product size (bp)
Eastern grey kangaroo	macF	GCATCCATCTTAATTCTCCTCA	250
Eastern grey kangaroo	marR	GGTTYTAGTATGTAGTTTTCAAA	250
Swamp wallaby	walF	GCCCTACTTTCATTAGCAC	425
Swamp wallaby	marR	GGTTYTAGTATGTAGTTTTCAAA	425
Common wombat	VUCytBF	AGCATTCATCGACCTACCCA	289
Common wombat	VUCytBR	TGTTTCTTTGTAGAGGTAGGAG	289
Fallow deer	DDCytBF	AGCAACCTTAACTCGATTCTTC	197
Fallow deer	DDCytBR	AGAGAAATAGGAATAGGATGCC	197
Red deer	CECytbF3	CGCAGACAAAATCCCCTTTCA	482
Red deer	CERUR	GTTTTCGATTGTGCTGGTGA	482
Sambar deer	RUCytbF4	CCAGTGCCTATTCTGAATCTTAGC	161
Sambar deer	CERUR	GTTTTCGATTGTGCTGGTGA	161

ISSN: 1756-3305