Fig. 3

SEA drives CD5 and PD-L1 expression of B cells in vitro. Splenic B cells and PerC washout cells from uninfected mice were cultured for 24 h in the presence of PBS or indicated stimuli. a Representative dot plots indicate the expression of CD5 on gated CD19⁺ B cells from spleen and peritoneal cavity and significance levels from statistical analyses are indicated. b Flow cytometric histograms represent cell surface expression of PD-L1 in splenic and PerC B cells, and significance levels from statistical analyses of PD-L1 MFI of splenic and PerC B-cell are indicated. The expression of CD23 (c) and CD21 (d) in splenic B cells was measured at the end of culture. The shaded histograms represent the FMO control. In e and f, splenic B cells were cultured for 24 h in the presence of PBS or SWA (20 μg/ml). e Representative dot plots indicate the expression of CD5 on gated CD19⁺ B cells from spleen and significance levels from statistical analyses are indicated. f Flow cytometric histograms represent cell surface expression of PD-L1 in splenic B cells, and significance from statistical analyses of PD-L1 MFI of splenic B-cell are indicated. All data are representative results of two independent experiments with at least 4 mice per group. *P < 0.05, **P < 0.01