Fig. 2

Structure comparison between FPPS of Leishmania and Giardia. Three-dimensional structure alignments based on the structural similarity of L. major FPPS crystallographic structure (PDB 4JZB, lilac chain A) with L. infantum (green) (a) and alignment of L. major FPPS (lilac) with G. duodenalis (yellow) (d). The inset highlights the residues (sticks) of FARM (b, e) and SARM (c, f) motifs in the catalytic site. Residues of both motifs, FARM and SARM, are conserved in genus Leishmania (b, c), and the residues of this region are not conserved between L. major and G. duodenalis (e, f). Electrostatic potential surface map generated by PyMol for the structures of FPPS (g). The color scale for surface electrostatic potential was set from − 1 kT/e (red) to 1 kT/e (blue). Key: white, neutral; blue, positive charge; red, negative charge