Fig. 3

The new flagellum emerges from its flagellar pocket during assembly. The full stack is shown at Additional file 2: Video S2. a Slice view of a trypomastigote cell with the basal body of the new flagellum, which is posterior in relation to the mature flagellum (MF). b Top view of the new flagellum emerging from its own flagellar pocket in a segmented cell. c Top view of internal organization of the segmented kinetoplast, new flagellum, mature flagellum and nucleus. The new flagellum is closely associated to the kinetoplast and is in a posterior position in relation with the mature flagellum. d Top view of the reconstructed cell body, the new flagellum is now observed at the cell surface. Abbreviations: NF, new flagellum; MF, mature flagellum; K, kinetoplast; N, nucleus. Notes: White and black arrowheads indicate the new and the mature basal bodies, respectively. Anterior region and posterior regions of the cell are indicated on panel a. Scale-bars: 1 µm