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Fig. 7 | Parasites & Vectors

Fig. 7

From: Timing and original features of flagellum assembly in trypanosomes during development in the tsetse fly

Fig. 7

Schematic illustration of flagellum assembly in trypomastigote cells and in transition forms from the PV. From left to right the first zoom with trypanosomes in dark grey represents the surface view of the posterior region, the emergence of the flagellum from the flagellar pocket whilst the second zoom with trypanosomes in light grey represents the internal view showing the full flagellum elongation process. At the top, a trypomastigote containing a round nucleus illustrates the stage that precedes flagellum assembly. The outside view shows a trypanosome where only the mature flagellum is visible outside of the cell body. At stage 1, the internal view represents the 24% of 2F1K1N trypomastigote cells with an oval nucleus and two signals for FTZC in IFA experiments. At stage 2, the short new flagellum is located inside the flagellar pocket whilst only the mature flagellum is visualized outside of the cell body. The internal view represents the 2F1K1N configuration of trypomastigote cells with an elongated nucleus, a new TZ and a new axoneme. The short new flagellum is linked by its tip to the side of the mature flagellum via the flagella connector as demonstrated by serial TEM sectioning. At stage 3, the new flagellum is associated to the mature flagellum via the flagella connector and elongates so that it is observed outside of the cell body. The internal view shows that the nucleus is closer to the kinetoplast indicating the migration towards the posterior region. Stage 4 are early transition form cells where the new flagellum is elongating as demonstrated in the outside view. In the internal view, the nucleus and the kinetoplast are at the same plane. Stage 5 corresponds to the late transition form. The outside view shows that the new flagellum has rotated in relation to the mature flagellum. The internal view shows nucleus migration is more advanced in a late transition form. At the bottom, a short and a long epimastigote cell are represented, resulting from the asymmetric division

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