Fig. 2
From: Revealing new tick-borne encephalitis virus foci by screening antibodies in sheep milk
Detecting TBEV antibodies in sheep milk. a The milk samples were screened for the presence of anti-TBEV-IgG antibodies using a commercial ELISA (Immunozym FSME IgG All Species; Progen Biotechnik GMBH, Heidelberg, Germany). Antibody titres > 126 VIEU/ml (dotted line) were considered positive, according to manufacturer’s instructions. b Western blot analysis of representative milk samples. BHK-21 cells were transfected with plasmid-encoding TBEV membrane and envelope (ME) proteins. Cell lysate was separated on sodium dodecyl sulphate polyacrylamide gel and transferred to nitrocellulose membrane. The milk samples were then used as primary antibody to detect the envelope (E)-protein of TBEV (TBEV-E ≈ 50 kDa). Non-transfected cells are denoted as mock. The expression levels were verified by GAPDH (≈ 35 kDa)