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Fig. 5 | Parasites & Vectors

Fig. 5

From: Revealing new tick-borne encephalitis virus foci by screening antibodies in sheep milk

Fig. 5

Stability of tick-borne encephalitis virus (TBEV) in sheep milk. TBEV is unstable at room temperature and at 37 °C. 100,000 focus forming units (Ffu) of TBEV were added per ml of DMEM (Dulbecco’s modified Eagle’s medium), milk and colostrum. Virus was incubated for 72 h at 4 °C (a), room temperature (RT) (b) or 37 °C (c), and viral titers were measured by means of focus forming assay, as previously described [15]. DMEM, milk and colostrum were incubated at 37 °C and pH was measured using pH-indicator strips (d). DMEM, milk and colostrum were treated with beta-propiolactone for 16 h at 4 °C for sterilisation, followed by incubation at 37 °C for 2 h to hydrolyse and inactivate beta-propiolactone. After sample treatment, 100,000 Ffu of TBEV were added, samples were incubated at 37 °C and pH (e) and Ffu (f) were measured at the indicated time points

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