Fig. 5

Results of gene rescue assays on mutant and transgenic Caenorhaboditis elegans strains. a–f The anatomical expression pattern in transgenic C. elegans strain transformed with Cel-daf-8p::Cel-daf-8::gfp.g–l The anatomical expression pattern in transgenic C. elegans strain transformed with Cel-daf-8p::Hco-daf-8::gfp.a–c, g–i are the DIC images of the rescued worms. d–f, j–l are the representative GFP expression patterns. Intense green fluorescent signals were detected in the intestine (i) head neurons (hn) nerve-ring (nr), excretory duct cells (ec) and tail neurons (tn). m Non-rescued mutant worms remain at the dauer stage at 25 °C with constrictive body and stationary pharynx. n, o Worms that developed to adult worms rescued successfully with the plasmids Cel-daf-8p::Cel-daf-8::gfp or Cel-daf-8p::Hco-daf-8::gfp at 25 °C, respectively. p Percentage of different phenotypes of worms that developed at 25 °C in the daf-8 C. elegans mutant strain (CB1393) and the C. elegans transgenic strains. ***P < 0.001. Scale-bars: 50 μm