Fig. 5

Dauer TGFβ signaling is downregulated during N. brasiliensis iL3 activation. a Predicted protein alignment of the ligand domains of DAF-7-like TGFβ ligands from N. brasiliensis (Nbr-DAF-7), H. contortus (Hco-DAF-7), N. americanus (Nam-DAF-7), and C. elegans (Cel-DAF-7). Gray indicates similar amino acids. b Transcript abundance was determined for the coding region of Nbr-daf-7 in iL3 treated with either Δ7-dafachronic acid (DA) or the vehicle control (ethanol) for 24 h at 20 °C, 26 °C or 37 °C. TMM-normalized transcript abundance was plotted as the mean counts per million (CPM) for each condition; error bars represent the SEM. Statistical significance was evaluated between 20 °C vehicle and 20 °C DA conditions and also between 20 °C vehicle and 37 °C vehicle conditions; *, fold-change > 2.0, FDR-adjusted P-value < 0.05. c Heat map depicting differences in transcript abundance in iL3 treated with either Δ7-dafachronic acid (DA) or the ethanol vehicle control (Veh) for 24 h at 20 °C, 26 °C or 37 °C. Transcript abundance was z-scaled for each gene; biological replicates are indicated with a, b or c