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Fig. 5 | Parasites & Vectors

Fig. 5

From: Angiostrongylus cantonensis Galectin-1 interacts with Annexin A2 to impair the viability of macrophages via activating JNK pathway

Fig. 5

Phosphorylation of MAPK signaling pathway proteins following AcGal-1 treatment. a Results of MAPK phospho-antibody microarray. The proteins outlined in a red box showed a significant increase in phosphorylation levels, including JNK and HSP27, and a decrease in pERK1/2, following AcGal-1 treatment. b The levels of pJNK and pERK1/2 were confirmed by western blotting. SP600125 was used to inhibit JNK phosphorylation, and cells treated with EGF were used as a positive control to reflect the EGFR/MAPK pathway activation. c Quantitation of expression intensity of each protein in b. d Western blot analysis of apoptosis/anti-apoptosis -related proteins in AcGal-1-treated or non-treated macrophages in the presence or absence of the JNK inhibitor SP600125 on macrophage apoptosis. e Quantitation of expression intensity of each protein in d. Data represent the mean ± standard deviation (SD; vertical bars) of triplicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001

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