Fig. 1

Molecular (a–d) and biological (e–h) changes induced by tetraspanin1(EgTSP1)-specific dsRNA in different developmental stages of Echinococcus granulosus using electro-soaking (ES) and electroporation (EP) compared to the untreated (Ctrl) and the irrelevant dsRNA (Ctrl-irr) controls. a EgTSP1 expression profile in different developmental stages of E. granulosus in vitro; invaginated protoscoleces (PSCi), evaginated protoscoleces (PSCe), one-proglottid worms (SW1) and three or more proglottids worms (SW3). b–d Suppression of EgTSP1 in protoscoleces (PSC), one-proglottid worms (SW1) and three or more proglottids worms (SW3) by dsRNA on days 3, 7 and 14. e Viability changes of protoscoleces treated with EgTSP1-specific dsRNA. f Evagination rate of protoscoleces treated with EgTSP1-specific dsRNA. g, h Changes in body contractions per minute in one-proglottid worms (SW1) and three or more proglottids worms (SW3) treated with EgTSP1-specific dsRNA. For Ctrl-irr EgTSP1 expression data are only demonstrated for electro-soaking method. Bars show the mean ± SE. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001