From: Rapid clearance of Borrelia burgdorferi from the blood circulation
PCR | Bp (flaB) | Bbss (ospA) | ||
---|---|---|---|---|
Working concentration | Reaction volume (µl) | Working concentration | Reaction volume (µl) | |
Master mixa | 1× | 10 | 1× | 10 |
Forward primer | 600Â nM | 1.2 | 900Â nM | 0.64 |
Reverse primer | 600Â nM | 1.2 | 900Â nM | 0.64 |
Probe | 200Â nM | 0.8 | 100Â nM | 0.97 |
Reference dyeb |  | 0.1 | – | – |
Nuclease-free water | Â | 4.2 | Â | 5.25 |
Template DNA | Â | 2.5 | Â | 2.5 |