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Table 2 PCR mixture used in this study

From: Rapid clearance of Borrelia burgdorferi from the blood circulation

PCR Bp (flaB) Bbss (ospA)
Working concentration Reaction volume (µl) Working concentration Reaction volume (µl)
Master mixa 10 10
Forward primer 600 nM 1.2 900 nM 0.64
Reverse primer 600 nM 1.2 900 nM 0.64
Probe 200 nM 0.8 100 nM 0.97
Reference dyeb   0.1
Nuclease-free water   4.2   5.25
Template DNA   2.5   2.5
  1. aQuantiNova probe PCR master mix (Qiagen, Hilden, Germany)
  2. bQN ROX reference dye (Qiagen)
  3. Abbreviations: Bbss, Borrelia burgdorferi sensu stricto; Bp, Borrelia persica