Skip to main content

Table 2 PCR mixture used in this study

From: Rapid clearance of Borrelia burgdorferi from the blood circulation

PCR

Bp (flaB)

Bbss (ospA)

Working concentration

Reaction volume (µl)

Working concentration

Reaction volume (µl)

Master mixa

1×

10

1×

10

Forward primer

600 nM

1.2

900 nM

0.64

Reverse primer

600 nM

1.2

900 nM

0.64

Probe

200 nM

0.8

100 nM

0.97

Reference dyeb

 

0.1

–

–

Nuclease-free water

 

4.2

 

5.25

Template DNA

 

2.5

 

2.5

  1. aQuantiNova probe PCR master mix (Qiagen, Hilden, Germany)
  2. bQN ROX reference dye (Qiagen)
  3. Abbreviations: Bbss, Borrelia burgdorferi sensu stricto; Bp, Borrelia persica