An immunoinformatic approach driven by experimental proteomics: in silico design of a subunit candidate vaccine targeting secretory proteins of Leishmania donovani amastigotes

Table 3 List of MHC II epitopes with characteristic affinity and coverage

Epitope no.	Selected HLA epitopes	Protein ID	Starting position	Vaxijen score	IEDB class II rank	IL-10	World TPC (%)	Epitope conservancy^a in other Leishmania spp. in RefSeq database	Self^b
H2-01	QDCKFVLVKAAAPAA	E9BDB8	325	0.77	6.12	No	98.71	L. major, L. infantum	No
H2-02	AAYYIKAAERIAAKG	E9BI76	321	0.94	4.67	No	97.15	L. major, L. infantum	No
H2-03	TFVKWNFTAFLVDKD	E9BI90	121	1.19	8.91	No	97.76	L. infantum	No
H2-04	LGTTFVKWNFTAFLV	E9BI90	118	0.97	9.34	No	97.46	L. infantum	No
H2-05	TTFVKWNFTAFLVDK	E9BI90	120	1.06	8.31	No	97.76	L. infantum	No
H2-06	LTKLFRYKSSRSESE	E9BKM5	486	0.82	6.18	No	95.45	L. major, L. infantum	No
H2-07	WLKGYFRLGVAMESM	E9BT68	71	1.01	7.98	No	99.32	L. major, L. infantum, L. mexicana	No
H2-08	APLMLYISKMVPTAD	E9BT80	376	1.10	3.24	No	93.09	L. major, L. infantum, L. mexicana, L. braziliensis, L. panamensis	No
H2-09	NTDFVMYVASVPSEG	P23223	194	1.10	8.86	No	90.42	L. infantum	No
H2-10	ASDAGYYSALTMAIF	P23223	335	0.89	5.58	No	98.63	None	No
H2-11	LVKYLIPQALQLHTE	P23223	143	0.85	4.67	No	98.72	L. infantum	No
H2-12	DILVKYLIPQALQLH	P23223	141	0.74	2.72	No	94.14	L. infantum	No
H2-13	SDAGYYSALTMAIFQ	P23223	336	0.70	9.81	No	99.53	None
H2-14	CNGGLMLQAFEWLLR	Q95WR6	188	1.04	8.42	No	94.48	L. infantum	No

ISSN: 1756-3305