Table 1 Plasminogen-binding proteins of AsL3C and AsL3ES identified by MS from analysed spots. All identifications belonged to A. suum proteins deposited in databases
Spot number | Antigenic extract | Accession code | Protein definition | Theoretical MW (kDa) | Theoretical pI | Molecular function | Biological process |
|---|---|---|---|---|---|---|---|
1 | AsL3C | ERG85667 | Maguk p55 subfamily member 6 | 128.3 | 7.8 | Binding activity | – |
12, 13 | AsL3C | Q05893 | Phosphoenolpyruvate carboxykinase (GTP) | 72.2 | 6.3 | Phosphoenolpyruvate carboxykinase (GTP) activity | Gluconeogenesis |
15, 16 | AsL3C | CAK18211 | Disulphide isomerase | 55.6 | 4.9 | Protein disulfide isomerase activity | Cell redox homeostasis |
18 | AsL3C | ERG82657 | Atp synthase subunit | 58.8 | 5.7 | Proton transmembrane transporter activity | ATP synthesis coupled proton transport |
20, 21, 49, 50 | AsL3C, AsL3ES | ERG85026 | Glucose-6-phosphate isomerase | 80.1 | 9.1 | Glucose-6-phosphate isomerase activity | Glycolytic process |
23, 24, 36 | AsL3C | ERG87158 | Actin-2 | 41.8 | 5.3 | Binding activity | Actin cytoskeleton organization |
26, 28, 29 | AsL3C | ERG79663 | Fructose-bisphosphate aldolase 1 | 39.1 | 7.1 | Fructose-bisphosphate aldolase activity | Glycolytic process |
31, 32 | AsL3C | ERG79426 | Glyceraldehyde-3-phosphate dehydrogenase | 38.5 | 7.7 | Glyceraldehyde-3-phosphate Dehydrogenase activity | Glycolytic process |
33, 34 | AsL3C | ERG81473 | 32 kda beta-galactoside-binding lectin | 31.8 | 6.4 | Carbohydrate binding | – |
35, 38 | AsL3C | ERG81802 | 60s ribosomal protein l23 | 14.8 | 10.5 | Structural constituent of ribosome | Translation |
41, 42 | AsL3ES | ERG86435 | Prion-like domain-bearing protein 25 | 141.5 | 7.3 | Catalytic activity | Carbohydrate metabolic process |
44, 45, 51, 52, 54, 55 | AsL3ES | ERG85099 | Tachykinin-like peptides receptor 99d | 87.8 | 4.9 | G protein-coupled receptor activity | – |