Fig. 1

TbVAB localizes to acidocalcisomes, lysosomes and endosomal vesicles in the bloodstream forms of Trypanosoma brucei. The endogenous TbVAB::YFP partially co-localized with endocytosed DyLight 594-lectin (a), the acidocalcisomes marker TbVP1 (b) and the recycling endosomal marker Rab11 (c) in BSF of T. brucei as shown by immunofluorescence microscopy. No colocalization with Mito-Tracker was observed (d). Nuclei and kinetoplasts were stained with 50 ng/ml DAPI. Scale-bar: 10 μm