Fig. 2

Growth curves of bloodstream forms of Trypanosoma brucei after depletion of TbVAB and TbVAA. a Cumulative growth curves of the inducible conditional TbVAB knockout in T. brucei bloodstream SmOx B427 cells. The ectopically TbVAB modified cells were grown in HMI-9 (10% FCS) with 1μg/ml tetracycline and used as a control. TbVAB expression was detected by Western blot analysis with the internal loading control being monoclonal antibody L8C4 which recognizes the paraflagellar rod. b Analysis of kinetoplast (K) and nucleus (N) numbers in the bloodstream form T. brucei after knockout of TbVAB. Kinetoplasts and nuclei of 510 cells in each group were counted after stained with DAPI. c Cumulative growth curve of the inducible knockdown of TbVAA in T. brucei bloodstream forms. Non-induced trypanosomes were used as control. The extent of TbVAA mRNA knockdown was assayed by qRT-PCR at indicated time points. The TbVAA mRNA expression from biological triplicates was shown with reference to the GAPDH gene