Fig. 6

Depletion of TbVAB renders trypanosome cells less sensitive to trypanolysis by normal human serum. a Western blot analysis detecting endocytosed TLF shows relative uptake in non-induced and TbVAB depleted cells after incubation with 5% NHS at indicated time points. L8C4 was used as a constitutive control. b Trypanosome cell density was measured after incubation with normal human serum for 8h or 16h. Induced TbVAB depletion cells and non-induced cells were subjected to incubation with NHS. Error bars represent the standard deviation of biological triplicates. The unpaired t-test indicated significant differences (**P < 0.01, ***P < 0.001) between non-induced and cKO induced cells