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Fig. 2 | Parasites & Vectors

Fig. 2

From: Australian Aedes aegypti mosquitoes are susceptible to infection with a highly divergent and sylvatic strain of dengue virus type 2 but are unlikely to transmit it

Fig. 2

Midsagittal sections showing tissue distribution of DENV QML16 and QML22 strain infection within orally inoculated Ae. aegypti. Mosquitoes were examined for the distribution of DENV infection by IFA analysis of paraffin sections using an anti-flavivirus Envelope protein monoclonal antibody and Alexa-fluor 488 conjugated secondary antibody for DENV (green) and DAPI staining for DNA (blue). a Example of whole-body staining of mosquitoes infected with DENV QML16 at 14 dpi showing staining in head (H), midgut (M); and salivary glands (S). b Example of whole-body staining of mosquitoes infected with DENV QML22 at 14 dpi showing infection limited to the midgut. No staining was observed beyond midguts for mosquitoes inoculated with QML22. Scale-bars: 1 mm

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