Fig. 1

Primary small-molecule screening identifies compounds that inhibit Toxoplasma growth. RH-2F-infected HFF cells were incubated with 5 μM each compound for 72 h. β-Gal activity was detected; and inhibition was calculated with DMSO as a negative control (0% inhibition) and 10 μM pyrimethamine as a positive control (100% inhibition). Furthermore, compounds that showed more than 70% parasite inhibition (68 compounds) were screened for their effects on host cell viability. Green triangles, no host cell toxicity; red rectangles, host cell toxicity; black circles, less than 70% parasite inhibition