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Fig. 4 | Parasites & Vectors

Fig. 4

From: Two old drugs, NVP-AEW541 and GSK-J4, repurposed against the Toxoplasma gondii RH strain

Fig. 4

Inhibitory effects of NVP-AEW541 and GSK-J4 HCl on parasite invasion. RH GFP-TgAtg8-infected HFFs were pre-treated with NVP-AEW541 (2.5 μM), GSK-J4 HCl (5 μM) or DMSO for 8 h, and then the tachyzoites were released and added to fresh HFFs to invade for 1 h. The cells were fixed without permeabilization and stained with DAPI and anti-SAG1 antibody. a Representative immunofluorescence micrographs of parasites treated with vehicle (DMSO), GSK-J4 HCl and NVP-AEW541, respectively. White arrowheads indicate the intracellular parasites. b Invasion events were scored via differential staining as GFP+SAG1− (invaded) and GFP+SAG1+ (extracellular). A total of 20 randomly selected fields were counted for each triplicate sample. Shown are the average values and standard deviations from one experiment representative of three independent experiments

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