Fig. 5
From: Two old drugs, NVP-AEW541 and GSK-J4, repurposed against the Toxoplasma gondii RH strain
Inhibitory effects of NVP-AEW541 and GSK-J4 HCl on intracellular parasite replication. a HFFs were treated with NVP-AEW541 (2.5 μM), GSK-J4 HCl (5 μM) or DMSO for 24 h following infection with RH GFP-TgAtg8 parasites for 2 h. The cells were fixed, and the number of parasites per vacuole was determined. In addition, representative immunofluorescence micrographs of intracellular parasites are shown in the corresponding bar chart. b Flow cytometric analysis of the effects of NVP-AEW541 and GSK-J4 HCl on cell cycle progression. The DNA contents of the parasites were measured by PI staining. c The percentage of parasites in the S/M and G1 phases of the cell cycle