Fig. 6

Protective efficacy of NVP-AEW541 and GSK-J4 HCl on acutely infected mice. a Effect of NVP-AEW541 and GSK-J4 HCl on the survival rate of infected mice. BALB/c mice were infected intraperitoneally (ip) with 100 RH strain tachyzoites. Beginning on the day of infection, each drug at 50 mg/kg, vehicle (DMSO) or positive drug (pyrimethamine) was orally administered for 5 days. These mice were observed for an additional 15 days, and the survival times of the infected mice were recorded for 20 days (n = 10 for each group). b–d Effect of NVP-AEW541 and GSK-J4 HCl on parasite burden in tissues of infected mice. At 7 dpi, qPCR analysis of infected brain (b), liver (c) and spleen (d) samples was performed by using the Toxoplasma B1 gene as an indicator of parasite burden. Error bars represent the averages and standard deviations for parasite burden among the mice examined (n = 5 per group)