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Table 1 Primers and probes used for Granada, Massilia and Arrabida detection

From: Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses

Name

Sequence (5′–3′)

Nucleotide positions

Primer_F

GAGCATGACATTAGCAGAGTTTCTGA

997–1022

Primer_Re

GTAGTCCCATTGCCAGCTTTCT

1102–1123

Ph_Probe

FAM-TGAGCTCTGATGAYATGTC-MGB-NFQ

1028–1046

IC_Probe

NED-CCAGCACACATGTGTCTACT-MGB-NFQ

  1. Notes: Positions are based on the complete sequence from GR25 virus (GenBank: GU135606)
  2. Abbreviations: MGB, minor groove binder; NFQ, non-fluorescent quencher