Table 3 Limit of detection and data variation Cq of the qRT-PCR assay
From: Real-time RT-PCR assay to detect Granada virus and the related Massilia and Arrabida phleboviruses
Virus | copies/µl | n/N (%) | Cq-values | |
|---|---|---|---|---|
Mean ± SD | Range | |||
GRV | 0.1 | 0/8 (0) | ND | |
1 | 7/8 (86) | 38 ± 0.4 | 38–39 | |
10 | 8/8 (100) | 36 ± 0.5 | 35–36 | |
ARRV | 0.1 | 0/8 (0) | ND | |
1 | 7/8 (86) | 37 ± 1.2 | 35–39 | |
10 | 8/8 (100) | 34 ± 0.2 | 34–35 | |
MASV | 0.1 | 0/8 (0) | ND | |
1 | 8/8 (100) | 37 ± 0.6 | 36–38 | |
10 | 8/8 (100) | 34 ± 0.4 | 34–35 | |