Table 1 Primers, annealing temperatures and product size
From: Genomic studies on Strongyloides stercoralis in northern and western Thailand
Region amplified | Primer | Sequence (5′–3′) | Annealing T (°C) | Product size (bp) | |
|---|---|---|---|---|---|
SSU HVR-I | Forward Reverse Sequencing | ZS6492 RS5402 ZS6492 | AAACATGAAACCGCGGAAAG CATTCTTGGCAAATGCTTTCG AAACATGAAACCGCGGAAAG | 50 | 825 |
SSU HVR-IV | Forward | 18SP4F | GCGAAAGCATTTGCCAA | 57 | 712 |
Reverse | 18SPCR | ACGGCCGGTGTGTAC | |||
Sequencing | ZS6269 | GTGGTGCATGGCCGTTC | |||
cox1 | Forward | ZS6985 | GGTGGTTTTGGTAATTGAATG | 47 | 837 |
Reverse | ZS6986 | ACCAGTYAAACCACCAATAGTAA | |||
Sequencing | ZS6990 | GGTTGATAAACTATAACAGTACC | |||