Genetic diversity and phylogenetic relationships of tsetse flies of the palpalis group in Congo Brazzaville based on mitochondrial cox 1 gene sequences

Table 1 Genetic diversity indices, test of selective neutrality, goodness-of-fit-test

Population	No. of sequences (No. of haplotypes)	S	Pi	Hd	Neutrality test		Goodness-of-fit
Population	No. of sequences (No. of haplotypes)	S	Pi	Hd	Tajima’s D	Fu’s Fs	SSD	Hrag
Bouemba	88 (9)	0.9882	0.0022	0.5815	− 1.13378 (P = 0.13)	− 3.22268 (P = 0.086)	0.00224 (P = 0.568)	0.05537 (P = 0.706)
Talangai	87 (21)	6.8209	0.0152	0.8062	− 2.76701 (P< 0.0001)	− 1.05260 (P = 0.420)	0.01219 (P = 0.778)	0.02424 (P = 0.866)
Bomassa	88 (25)	7.0867	0.0158	0.8602	− *2.64837 (P <0.0001)*	− 2.86023 (P = 0.2180)	0.02735 (P = 0.178)	0.06618 (P = 0.115)

Note: Tajima’s D, significance of P < 0.01 is highlighted in bold. Number of haplotypes and Tajima’s D, Fu’s Fs, SSD and Hrag P-values are in parentheses
Abbreviations: S, number of segregating sites; Pi, nucleotide diversity; Hd, haplotype diversity; SSD, sum of squared deviation between the observed and expected distribution of pairwise differences; Hrag, Harpending’s raggedness index (non-significant, data have relatively good fit to a model of population expansion) and a ragged distribution suggests that the lineage was widespread