Divergent domains of 28S ribosomal RNA gene: DNA barcodes for molecular classification and identification of mites

Table 2 Universal or degenerate primers for divergent regions of 28S rDNA for the nine mite species

Gene fragment	Length (bp)	Primers (5’-3’)	Tm (°C)
D2	532	F: AACAAGTACHGTGAKGGAA	52
D2	532	R: CTCCTTGGTCCGTGTTTC	52
D3	252	F: GTCTTGAAACACGGACCAA	54
D3	252	R: ATCGATTTGCACGTCAGAA	54
D5	419	F: TTCTGACGTGCAAATCGAT	55
D5	419	R: GGCAGGTGAGTTGTTACACA	55
D6	202	F: GTGTGTAACAACTCACCTGCC	55
D6	202	R: TTGCTACTACCACCAAGATCTG	55
D7	465	F: CAGATCTTGGTGGTAGTAGCA	55
D7	465	R: CCTTGGAGACCTGCTGC	55
D8	314	F: GCAKCAGGTCTCCAAGG	52
D8	314	R: GTTTTAATTAGACAGTCGGATTC	52

ISSN: 1756-3305