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Table 3 Sequencing results of the six divergent regions of 28S rDNA for the nine mite species

From: Divergent domains of 28S ribosomal RNA gene: DNA barcodes for molecular classification and identification of mites

DD Mite species Length SR% GenBank ID CRS GenBank ID I/C
D2 D. farinae 450–542 20.0 (2/10) MH540331–32 D. farinae JQ000555 98/100
P. cuniculi 533 40.0 (4/10) MH540338–41 P. ovis JQ000549 98/100
S. canis 465–550 10.0 (1/10) MH540342 Mesalgoides sp. JQ000537 77/100
O. bacoti 465 100 (5/5) MH540333–37 O. bursa FJ911789 92/100
C. malaccensis 617 10.0 (1/10) MH540329 H. simplex KP325024 99/100
D. folliculorum / 0 (0/20)
D. brevis / 0 (0/20)
D. canis / 0 (0/20)
D. caprae 773 5.0 (1/20) MH540330 H. holopus KY922056 74/34
D3 D. farinae 252 50.0 (5/10) MH001311–15 D. farinae JQ000555 99/100
P. cuniculi 252–256 40.0 (4/10) MH001324–27 P. ovis JQ000549 99/100
S. canis 256 0 (0/10)  
O. bacoti 256 100 (5/5) MH001318–22 C. haematophagus FJ911790 99/100
C. malaccensis 312–313 50.0 (5/10) MH001306–10 Oudemansicheyla sp. KP276422 76/100
D. folliculorum 0 (0/20)
D. brevis 358–362 20.0 (4/20) MH540325–28 D. folliculorum KY922058 78/96
D. canis 368 10.0 (2/20) MH540323–24 D. folliculorum KY922058 86/95
D. caprae 0 (0/20)
D5 D. farinae 419 50.0 (5/10) MH001255–59 D. farinae JQ000555 100/100
P. cuniculi 419 60.0 (6/10) MH001275–80 P. ovis JQ000549 100/100
S. canis 413–423 30.0 (3/10) MH001267–69 Cystoidosoma sp. JQ000474 97/100
O. bacoti 413 100 (5/5) MH001260–64 Melicharidae sp. KP276394 99/100
C. malaccensis 436 100 (5/5) MH001270–74 Oudemansicheyla sp. KP276422 93/100
D. folliculorum 456 83.3 (5/6) KY305302–05, KY347877 D. folliculorum KY347877 99/100
D. brevis 438–442 75.0 (6/8) KY305306–11 D. brevis HQ718592 98/100
D. canis 445–459 85.7 (6/7) KY305312–17 D. folliculorum KY347877 93/100
D. caprae 502–507 71.4 (5/7) KY305318–22 D. folliculorum KY347877 83/100
D6 D. farinae 202 40.0 (2/5) MH001286, MH001290 D. farinae JQ000555 99/100
P. cuniculi 192–200 40.0 (2/5) MH001296, MH001300 P. ovis JQ000549 99/100
S. canis 195–202 20.0 (1/5) MH001301 C. amazonae KP325029 93/100
O. bacoti 195 83.3 (5/6) MH001291–95 Melicharidae sp. KP276394 98/100
C. malaccensis 195–198 40.0 (4/10) MH001281–84 Oudemansicheyla sp. KP276422 93/100
D. folliculorum 241 100 (6/6) KY305302–05, KY347876–77 D. folliculorum KY347876 99/100
D. brevis 232 75.0 (6/8) KY305306–11 D. brevis HQ718592 99/100
D. canis 201 85.7 (6/7) KY305312–17 D. brevis HQ718592 85/100
D. caprae 228–230 71.4 (5/7) KY305318–22 D. brevis HQ718592 90/100
D7 D. farinae 450–460 33.3 (2/6) MH001334,36 D. farinae JQ000555 100/100
P. cuniculi 450–468 30.0 (3/10) MH001343, MH001346, MH001347 P. ovis JQ000549 100/100
S. canis 450–501 30.0 (3/10) MH001349–51 A. ocelatus JQ000623 89/100
O. bacoti 450 50.0 (5/10) MH001338–42 Laelapidae sp. KP276392 96/100
C. malaccensis 450 0/15
D. folliculorum 450 0/15
D. brevis 450 0/15
D. canis 450 0/15
D. caprae 450 0/15
D8 D. farinae 314 100 (5/5) MH001240–44 D. farinae JQ000555 100/100
P. cuniculi 314 100 (5/5) MH001250–54 P. ovis JQ000549 100/100
S. canis 302–322 30.0 (3/10) MH001229, MH001230, MH001234 C. amazonae KP325029 87/100
O. bacoti 302 100 (5/5) MH001245–49 Laelapidae sp. KP276392 95/100
C. malaccensis 393 50.0 (5/10) MH001235–39 Oudemansicheyla sp. KP276422 74/100
D. folliculorum 519 100 (5/5) KY305323–27 D. folliculorum HQ728004 100/100
D. brevis 428–432 85.7 (6/7) KY305328–33 D. folliculorum HQ728004 96/92
D. canis 541–543 100 (5/5) KY305334–38 D. canis HQ728002 99/100
D. caprae 516 100 (6/6) KY305339–44 D. folliculorum HQ728004 86/100
  1. Abbreviations: DD, divergent domains; SR, sequencing success rate; CRS, closely related species; I/C, identity/coverage; D. farinae, Dermatophagoides farinae; P. cuniculi, Psoroptes cuniculi; S. canis, Sarcoptes scabiei canis; O. bacoti, Ornithonyssus bacoti; C. malaccensis, Cheyletus malaccensis; D. folliculorum, Demodex folliculorum; D. brevis, Demodex brevis; D. canis, Demodex canis; D. caprae, Demodex caprae; P. ovis, Psoroptes ovis; O. bursa, Ornithonyssus bursa; H. simplex, Haplochthonius simplex; H. holopus, Harpypalpus holopus; C. haematophagus, Chiroptonyssus haematophagus; C. amazonae, Chirnyssoides amazonae; A. ocelatus, Amerodectes aff. ocelatus