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Table 2 Seroprevalence of N. caninum antibodies in South American camelids

From: Toxoplasma gondii and Neospora caninum infections in South American camelids in Switzerland and assessment of serological tests for diagnosis

Country

Region

SAC species

Test (commercial provider)

Cut-off

No. positives/No. examined

% Positives

Reference

Peru

Highlands, Central and Southern Peru

Alpaca

IFAT

conjugate: anti-llama IgG FITC (VMRD)

1:50

39/92

42.4

[58]

 

Llama

 

1:50

39/212

18.4

 

Peru

Highlands, different regions

Alpaca

IFAT

conjugate: anti-llama IgG–FITC (VMRD) + confirmation of positive results by Westernblot

1:50

14/78

17.9

[59]

 

Llama

  

17/73

23.3

 

Peru

Puno, South-Eastern Peru

Alpaca

Immunoblot N. caninum

 

17/675

2.5

[30]

 

Llama

  

1/81

1.2

 
 

Vicuna

  

0/114

0

 

Peru

Yauli, Central Sierra

Alpaca

IFAT

conjugate: anti-llama IgG FITC (VMRD)

1:100

5/175

2.9

[72]

Peru

Central and South Peruvian Andes

Alpaca

IFAT

conjugate: anti-llama IgG FITC (VMRD)

1:100

425/2874

14.8

[57]

 

Llama

  

153/1845

8.3

 

Peru

Arequipa, South Peruvian Andes

Vicuna

ELISA, Chekit Neospora – cELISA (IDEXX) + confirmation of positive results by Westernblot

 

2/207

1.0

[73]

 

Alpaca

  

2/571

0.3

 
 

Llama

  

0/43

0

 

Peru

Huancavelica

Llama

IFAT

conjugate: anti-llama IgG FITC (VMRD)

1:100

12/98

12.2

[56]

Peru

Huancavelica

Alpaca

IFAT

1:100

47/288

16.3

[55]

Argentina

Jujuy

Llama

IFAT

conjugate: anti bovine IgG FITC (Sigma)

1:50

3/308

1.0

[37]

Australia

South

Alpaca

ELISA, N. caninum Antibody Test Kit ELISA (IDEXX) + Protein G conjugate (Zymed Recombinant Protein G HRP Conjugate, Invitrogen)

 

0/182

0

[53]

Australia

New South Wales and Victoria

Alpaca

ELISA, N. caninum Antibody Test Kit – cELISA (VMRD)

%I ≥ 30%a

3/100

3.0

[24]

Germany

Hesse

Alpaca

Immunoblot N. caninum

 

0/12

0

[30]

 

Llama

  

0/20

0

 

Czech Republic

Alpaca

ELISA, N. caninum Antibody Test Kit – cELISA (VMRD)

%I ≥ 30%a

0/1

0

[42]

 

Llama

  

1/8

12.5

 
 

Alpaca

IFAT

conjugate: anti-llama IgG FITC (VMRD)

1:50

0/1

0

 
 

Llama

  

1/8

12.5

 
  1. cELISA: competitive-inhibition enzyme-linked immunosorbent assay, IFAT: indirect fluorescent antibody test; a %I (% inhibition) calculated according the formula: %I = 100 − (OD sample × 100/mean OD negative control), OD optical density, S/P (%) calculated according to the formula: S/P = (OD sample/OD positive control) × 100; FITC: fluorescein isothiocyanate conjugate